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. 2014 Feb 7;8(Suppl-1):3–15. doi: 10.2174/2213988501408010003

Fig. (5).

Fig. (5)

Dose response to wortmannin and HC in Clone 10 cells. Each point represents Mean ± SD for 6 replicates. HC inhibits the lysosomal degradation of the autophagosomes so that more accumulate for imaging with increasing doses of HC. Wortmannin IC50 was similar at 5 and 10 μM HC (1.4 nM). A dose of 33 nM for wortmannin and 10 μM for HC was selected to produce a good signal: background and best Z’.