Fig. (9).

Compound qualification: example images (20x) of cells after treatment with compounds excluded through the secondary data analysis; Green – EGFP channel fluorescence, Red – nuclear channel fluorescence. (A): Positive control (0.3% DMSO); (B): Negative Control (33 nM wortmannin); (C): Fluorescent compound; (D): EGFP quenching compound; (E): very toxic compound; (F): somewhat toxic compound. Data associated are shown in Table 2. (G): Re-analysis of primary screen image data to remove fluorescent compounds. Compounds were removed that increased the normalized Dmrk (average nuclear diameter) or Icyt (average intensity in cytoplasmic region) values by more than 3 SDs from mean values (Mean = 0.06±6.05 – horizontal lines).