Table 2.

Example Data for Filtering Compound Interference Using 4 Features

Compound	% Pos (Norm)	Icyt (Norm)	Dmrk (Norm)	Npass (Raw)
Negative Control (0.3% DMSO)	-0.2	1.6	0.7	597
Positive Control (Wortmannin 33 nM)	101.3	-5.4	1.1	577
Fluorescent compound	94.6	56.1	42.9	631
GFP quenching compound	43.5	-73.8	-4.8	627
Very toxic compound	94.5	-48.3	-26.2	153
Somewhat toxic compound	107.2	-46.3	-17.0	551

‘Norm’ values are normalized to the mean of all plate wells; N Pass (Raw) is cell number in 4 fields of view