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. 2014 Mar;24(3):522–534. doi: 10.1101/gr.162537.113

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© 2014 Sakurai et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genome.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

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Figure 2. — Sequence statistics of ICE-seq. (A) Numbers of mapped tags used for ICE-seq in CE−, CE+, and CE++ conditions. The reference genome and cDNA sequences used here are hg18 and the UCSC gene, respectively. (B) Distribution histogram of gene expression estimated by the read coverage. Gene expression represented by the Log₂(RPKM) value for the reference transcriptome sequence is compiled at 0.1 Log₂(RPKM) intervals under conditions of CE− (blue), CE+ (yellow), and CE++ (magenta). (C) Pie chart of gene expression estimated by the read coverage. A total of 63% of human genes are detected with more than 20 times coverage (∼1 RPKM). (D) Scatter plots of logarithmic RPKM values in CE− versus CE+ or CE++. Their coefficients of determination (R²) are 0.998 and 0.995, respectively.