Table II.
The maturation rate of d-COC oocytes in melatonin containing-media
|
Melatonin
concentrations |
No. of d-COC |
Nuclear statues after 24 h (Mean±SE)
|
|||
|---|---|---|---|---|---|
| GV [n (%)] | GVBD [n (%)] | M-I [n (%)] | M-II [n (%)] | ||
| 100 M | 122 | 0 | 106 ± 13.9 (86.88)a | 97 ± 11 (79.5)a | 97 ± 10.4 (79.5)a |
| 0 (control) | 75 | 0 | 58 ± 6.2 (77.33) | 56 ± 6.4 (74.66) | 52 ± 5.9 (69.33) |
| 0 (vehicle) | 98 | 0 | 76 ± 8.4 (77.55) | 75 ± 7.9 (76.53) | 70 ± 6.9 (71.42) |
| 10 nM | 111 | 0 | 90 ± 9.7 (81.08) | 87 ± 8.9 (78.37) | 82 ± 7.9 (73.87) |
| 100 nM | 90 | 0 | 74 ± 7.6 (82.22) | 67 ± 6.7 (74.44) | 67 ± 6.9 (74.44) |
| 1 M | 90 | 0 | 71 ± 8.4 (78.88) | 58 ± 6.5 (64.44) | 58 ± 6.1 (64.44) |
| 10 M | 115 | 0 | 104 ± 14.8 (90.43)a | 97 ± 10.2 (84.34)a | 97 ± 10.9 (84.34)a |
GV: germinal vesicle, GVBD: germinal vesicle break down, M-I: metaphase-I, M-II: metaphase-II.
a within the same column, values with different letters were significantly different in comparison to control group. a) p < 0.004.
Chi-square test.