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. 2013 Jan;11(1):53–60.

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Toluidine Blue staining of spermatozoa. TB⁺ indicates sperm cells with abnormal chromatin and TB^- indicates sperm cells with normal chromatin (×100 eyepiece magnification).