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. 2013 Nov;11(11):905–912.

Figure 2.

The expression of VASA and SCP3 with addition of RA.

Figure 2

The results demonstrated that there is little or no expression of VASA and SCP3 in undifferentiated hESCs and differentiated EBs for 1 day. In contrast, EBs treated with RA demonstrated a dramatic increase in expression of the germ cell markers. The expression of VASA and SCP3 increased 57 and 29 fold respectively in the presence of 10 μM RA, compared with 1 μM RA.

undiff: undifferentiated hESCs; R0: the absence of RA; R1: 1μM RA; R3: 3μM RA; R5: 5μM RA; R7: 7μM RA; R10: 10μM RA.

Statistically significant differences (p < 0.05) are indicated by different letters (e, f).