Table    I .

Primers used for real time-PCR

Gene	Primer sequences	Fragment size (bp)	Annealing temperature ( o C)	Fluorescence measurement temperature ( o C)
VASA	5’-TCTGCGAAACATAGGGGATGA-3’	315	60	85
	5’-CTGCCAGTATTCCCACAACGA-3’
SCP3	5’-TGCAGAAAGCTGAGGAACAAG-3’	249	62	86
	5’-CTTGCTGCTGAGTTTCCATCA-3’
GDF9	5’-CGCAGAGGTCAGGAAACTGTC-3’	315	60	87
	5’-GGCAGGTACACATGACGGTCT-3’
TEKT1	5’-AGGCCATCCTTGACCAAGAAG-3’	236	62	89
	5’-TTTGACCTGGATCTCCTCCTG-3’
OCT-4	5’-ACATCAAAGCTCTGCAGAAAGAACT-3’	127	60	86
	5’-CTGAATACCTTCCCAAATAGAACCC-3’
β-actin	5’-GAGCTACGAGCTGCCTGACG-3’	416	60	91
	5’-CCTAGAAGCATTTGCGGTGG-3’