(A) (Top) Autoradiograph showing the 32P phosphate incorporation into wild-type ACV (lane 1) and the ACV S676A mutant (lane 2). (Bottom) ACV expression levels were monitored by immunoblot.
(B) Results were quantified by densitometry, and data are presented as the mean ± SEM.
(C) Graph of the levels of intracellular cAMP (pmol) in HEK293 cell lysates expressing the GαSQ227L constitutively active mutant. Data from cells expressing wild-type ACV (black) and the ACV S67A mutant (gray) are indicated. Data are representative of three separate experiments and are presented as mean ± SEM.