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. Author manuscript; available in PMC: 2014 Mar 4.
Published in final edited form as: Curr Biol. 2009 Feb 12;19(4):277–286. doi: 10.1016/j.cub.2009.01.039

Figure 4. DrpB is required for the transport of secretory proteins.

Figure 4

(A, B) Immunofluorescence analysis of dd-DrpBDN-parasites grown in presence and absence of Shld-1 over night and stained with the indicated antibodies. (C) Immunofluorescence analysis of Shld-1 treated parasites expressing dd-DrpBWT and dd-DrpBDN for 24 hours in presence of Shld-1 and stained with indicated antibodies. Whereas expression of dd-DrpBWT does not interfere with the normal staining pattern of the endosomal associated compartments, in case of dd-DrpBDN expression the respective protein appears to be secreted into the lumen of the PV. Scale bar: 10 μm (D) Expression of dd-DrpBDN does not influence pro-peptide processing in intracellular parasites. For the immunoblot parasites were allowed to grow for 36 hours in presence or absence of Shld-1, before lysate was prepared and probed with the indicated antibodies. Note that a weaker signal for the mature forms of MIC6 (45kDa) and M2AP (40 kDa) were detected in Shld-1 treated dd-DrpBDN parasites (−/+ Shld-1; molecular weights are indicated). DrpB is always shown in green colour in merged images.