Effect of ONX 0912 and bortezomib on chaperone proteins. A: Western analysis of chaperone proteins in liver from bortezomib, ONX 0912, and control mice. Liver CBS activity for each extract is also shown. Mice are classified as either R (responder) or NR (non-responder) based on CBS activity level. Control mice include a Tg-I278T Cbs−/− mice treated with vehicle (CMC), a Tg-hCBS CBS −/− mouse that was uninduced for CBS expression (No Zn Tg+ −/−), and a mouse that expresses endogenous levels of mouse CBS (No Zn Tg+ +/+). B: Immunoprecipitation experiment examining interaction of Hsp90, Hsp70, Hsp40 and Hsp27 with CBS. Extracts from a Tg-I278T Cbs−/− responder (Tg-I278T-R), Tg-I278T Cbs−/− non-responder (Tg-I278T-NR) and a Tg-hCBS Cbs−/− mouse, all treated with ONX 0912, were used. Lane “I” shows input extract; lane “+” shows material brought down with a-CBS antibody conjugated to beads; lane “−“ shows material brought down by beads alone.