Figure 2.

Immune responses induced in goats vaccinated with Ad or FP expressing PPRV glycoproteins. One goat was vaccinated intra-muscularly with a mixture of 1 × 10⁹ IU of Ad-H and 1 × 10⁹ IU Ad-F and another goat was vaccinated intra-muscularly with a mixture of 1 × 10⁸ pfu of FP-H and 1 × 10⁸ pfu FP-F. Goats were boosted intra-muscularly with the same doses of the respective vaccines, 5 weeks later. (A) Neutralising antibody titres were determined by 50% plaque reduction assay. (B) At post mortem examination, 9 weeks after vaccination, F- and H-specific T-cell responses in prescapular lymph nodes were analysed by flow cytometry of lymphocytes stimulated with Ad-H, Ad-F, FP-H, FP-F or control Ad and FP expressing an irrelevant antigen. Cells were stained for CD8 and intracellular IFNγ. Results are expressed as the percentage of CD8⁺ IFNγ⁺ T-cells following stimulation with virus vector expressing F or H minus percentage following stimulation with control virus vectors. Dot plot showing CD8⁺ IFNγ⁺ T-cells from the prescapular lymph node from the Ad-vaccinated goat after stimulation with Ad-H (C) and after stimulation with Ad expressing an irrelevant antigen (D).