Figure 4.

Effect of virus-vectored ovine IL-2 and/or GMCSF on T-cell responses induced in goats vaccinated with Ad expressing the H protein of PPRV. Goats were vaccinated intra-muscularly with 1 × 10⁹ IU of each Ad virus as described in Table 1 and were challenged intranasally with 1 × 10⁵ TCID₅₀ of pathogenic PPRV, Ivory Coast/89, 15 weeks later. PBMCs were stimulated with H peptides and H-specific T-cell proliferative responses were measured by ³H-thymidine incorporation (A); and H-specific CD8⁺ IFNγ⁺(B) and CD4⁺ IFNγ⁺(C) cells were analysed by flow cytometry. Results are expressed as the mean stimulation index or mean % IFNγ-containing cells ± S.E. of 3 to 4 goats.