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. 2014 Feb 9;2014:289327. doi: 10.1155/2014/289327

Figure 2.

Figure 2

Resveratrol (RSV) attenuated high glucose- (HG-) induced plasminogen activator inhibitor (PAI-1) expression and cell proliferation in rat mesangial cell (RMC), which might be Akt/nuclear factor-kappa B (NF-κB) pathway dependent. RMCs were cultured in DMEM containing 5.6 mM glucose (NG) and 10% FBS. When the confluence reached at 60%–70%, the medium was replaced with NG and 0.2% BSA. 24 h later, the cells were pretreated with 10 μM LY294002, 1 μM MK-2206, or an equal volume of DMSO for 30 min and then incubated for another 24 h with or without HG (25 mM) in the presence or absence of RSV (25 μM). After that, cells were collected and protein levels of PAI-1 ((a), (b)), Akt, p-Akt ((c), (d)), and NF-κB ((e), (f)) were detected using Western blotting assay. Additionally, cell proliferation was examined with CCK8 assay and BrdU incorporation, respectively. Results represent as mean ± SD. *P < 0.05 compared with NG, # P < 0.05 compared with HG.