Skip to main content
. 2014 Feb 28;14:141. doi: 10.1186/1471-2407-14-141

Figure 7.

Figure 7

Phospho-aspirin-2 induces oxidative stress in TNBC. A: PA-2 induced RONS in MDA-MB-231 and BT-20 cells after 1h treatment, as determined by DCFDA, DHE and MitoSOX Red staining and flow cytometry. B: Left panel: GSH level was suppressed in MDA-MB-231 cells treated with various concentrations of PA-2 for 24 h, BSO as a positive control. Values are mean ± SEM. Middle panel: PA-2 and BSO synergistically induced RONS. RONS production was determined by DCFDA staining in MDA-MB-231 cells treated with PA-2 or PA-2 plus BSO for 1 h. Right panel: PA-2 and BSO synergistically inhibited cell growth. Cell growth inhibition was determined by MTT in MDA-MB-231 cells treated with PA-2 or PA-2 plus BSO for 24h. C: PA-2 increased the levels of 15-F2t-Isoprostane in 24-h urine from nude mice bearing orthotopic MDA-MB231 (p < 0.05) and subcutaneous BT-20 xenografts (p < 0.006), while aspirin had no effect. Urinary 15-F2t-Isoprostane was determined using an ELISA kit, as described in Methods.