Figure 9.

Effect of high-fat diet or high-fat diet and low-dose streptozotocin on innervation of subbasal layer using isolated intact cornea. Innervation of the subbasal layer of cornea was determined by examining expression of corneal nerves following isolation of the intact cornea (whole mount) and staining with anti-tubulin as described in the Methods section. A representative image of the inferior whorl region of the cornea from a control rat (A), high-fat–fed rat (B), and diabetic rat (C) and periphery of the cornea from a control rat (D), high-fat–fed rat (E), and diabetic rat (F) is shown.