Table 3.
PCR primers used to detect survival of MAP mutant vaccine strains.
| Mutant Strains | JDIP ID# | Name | Sequence (5′-3′) | Length | ORF | ORF | POI | WT amplicon | Mutant amplicon |
|---|---|---|---|---|---|---|---|---|---|
| 315/STM68 | jdip005-F | MAP1566 Forward | GCTCTAGAGCTGGCATCAGGGCACTCAAGAAA | 32 | MAP1566 | 1074 | 1032 | 1179 | 3700 estimated |
| jdip005-R | MAP1566 Reverse | CCCAAGCTTGGGTATTCGCTGCACAGCATGTCAGGT | 36 | ||||||
| 316/2E11 | jdip006-F | 2E11-IP-F | GCTGCAGCAACCAGCCGA | 18 | FadE51 | 1836 | 137 bp upstream | 1845 | 5241 |
| jdip006-R | 2E11-IP-R | CCACCGTCACCGCAGGTAGA | 20 | MAP36951 | 1074 | 30 bp upstream | |||
| 319/30H9 | jdip009-F | MAP1566 Forward | GCTCTAGAGCTGGCATCAGGGCACTCAAGAAA | 32 | MAP1566 | 1074 | 272 | 1179 | 4575 |
| jdip009-R | MAP1566 Reverse | CCCAAGCTTGGGTATTCGCTGCACAGCATGTCAGGT | 36 | ||||||
| 318/40A940A9 | AMT152 | TTGCTCTTCCGCTTCTTCT | 19 | MAP0282c | 792 | N/A | N/A | N/A | |
| (sequencing primer) | MAP0283c2 | 513 | |||||||
| 329/fabG2_2* | jdip019_R1 | AMT 38 | GTA ATA CGA CTC ACT ATA GGG CNN NNC ATG | ||||||
| jdip019_R1 | AMT 858 | TGC AGC AAC GCC AGG TCC ACA CT | |||||||
| jdip019_R2 | AMT 39 | TAA TAC GAC TCA CTA TAG GG | |||||||
| jdip019_R2 | AMT 859 | CTC TTG CTC TTC CGC TTC TTC TCC |
1
These genes are encoded in opposite directions, away from the POI.
2
Transposon lost upon further replication.
*
Shin et al. (2006).