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HG concentrations and Gas6/Axl, ICAM-1 and VCAM-1 expression in HMEC-1 s. HMEC-1 s were cultured in 5, 20 and 40 mM glucose and equimolar concentrations of mannitol for 24 h. (A,B) Gas6/Axl mRNA and Axl proteins expression were decreased by HG. (C) The expressions of ICAM-1 and VCAM-1 proteins were increased with a glucose dose-dependent manner. HMEC-1 s grown in mannitol did not change in Gas6/Axl, ICAM-1 and VCAM-1 expression.
