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. 2013 Oct 26;35(3):703–713. doi: 10.1093/carcin/bgt356

Fig. 4.

Fig. 4.

ERα is required in the regulation of BNF-mediated p21Cip1/Waf1 expression. For A, B and C, MCF-7 cells were transfected with 40nM negative or ERα siRNA for 36h, then treated with 10 µM BNF for 36h in A and B, (A) Western blot analysis of p21Cip1/Waf1 protein ERK phosphorylation, (B) qPCR analysis of p21Cip1/Waf1 mRNA. (C) qPCR analysis of p21Cip1/Waf1 mRNA in BNF-treated MCF-7 cells between negative siRNA and ERα siRNA group. (D) Western blot analysis of ERα protein in MCF-7 cells pretreated with 10 µM MG132 for 1h and then treated with 10 µM BNF or 10nM TCDD for 24h. **P < 0.01 and ***P < 0.001, versus DMSO-treated cells with negative siRNA; $, P < 0.05 and $$$, P < 0.001 versus DMSO-treated cells with ERα siRNA; ###, P < 0.001, versus BNF-treated cells with negative siRNA.