Fig. 5.
The effect of BNF on MCF-7 cell cycle depends on AhR. For A, B and C, MCF-7 cells were transfected with 40nM negative or AhR siRNA for 36h, then treated with 10 µM BNF for 36h. (A) Western blot analysis of the phosphorylation of AKT and ERK1/2, p21Cip1/Waf1, CYP1A1 and ERα protein. (B) qPCR analysis of p21Cip1/Waf1 and CYP1A1 mRNA. (C) Flow cytometric analysis of cell cycle arrest. *P < 0.05, **P < 0.01 and ***P < 0.001, versus DMSO-treated cells with negative siRNA; $$$, P < 0.001, versus DMSO-treated cells with AhR siRNA #, P <0.05 and ###, P < 0.001, versus BNF-treated cells with negative siRNA.