Figure 1.

Micro RNA (miRNA) analysis and determination of function. Human skeletal muscle samples obtained during independent or combined amino acid and exercise interventions analyzed for miRNA expression using (1) standard RNA isolation extraction methods, (2) microarray, (3) real-time quantitative polymerase chain reaction (qPCR) and Northern blotting; function determined and verified using (4) bioinformatics prediction software and (5) qPCR and protein detection methodologies (e.g., Western blotting).