Table 1. Effect of Freezing Time on the Particle Size of Liposome (n = 3).

Formulation	Freezing time, min	Particle size, nm	Polydispersity index (PDI)
F1	4	157.66 ± 0.57	2.5 ± 0.0
F2	8	158.66 ± 0.57	2.58 ± 0.14
F3	10	286.33 ± 2.88	2.66 ± 0.14
F4	45	36430 ± 1.25	1.41 ± 0.94
F5	> 60	290000 ± 6.65	2.05 ± 0.42