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. 2014 Feb 24;11:35. doi: 10.1186/1742-2094-11-35

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Copyright © 2014 Li et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Dose-dependent uptake of apoptotic bodies by MECs. (A) Ben-Men-I cells were incubated with CFSE-labeled apoptotic U-937 cells for 4 h at a MEC:apoptotic body ratio of 1:0, 1:1, 1:5, 1:7, 1:10, and 1:15. CFSE-fluorescence was analyzed using flow cytometry. The bar graphs (B) depict the percentage of CFSE-positive MECs (left graph) and the mean fluorescence intensity (MFI - right graph) of three independent experiments with the error bars representing SD. Statistical significance was analyzed by one-way ANOVA. Pair-wise Student’s t-test revealed P <0.001 adjusted according to Holm for all comparisons (marked ***) except where noted otherwise (** P <0.01).