Table 1.
miR-34a , miR-34b/c and DAPK1 MSP Primer sequences and the reaction condition
| Gene | Forward primer (5’ to 3’) | Reverse primer (5’ to 3’) | Tm/cycles/MgCl 2 | References |
|---|---|---|---|---|
|
miR-34a
|
|
|
|
|
| U-MSP |
GGGGATGAGGATTAGGATTTT |
CAAACAAAACACATAAAAACAACA |
58°C/35×/1.5 mM |
[23] |
| M-MSP |
GGGGATGAGGATTAGGATTTC |
ACAAAACGCATAAAAACGACG |
58°C/35×/1.5 mM |
|
|
miR-34b/c
|
|
|
||
| U-MSP |
TTTTTATTTGTTTTGTTTTGTGTTTGTTTTG |
CAACTACAACTCCCAAACAATCC |
56.5°C/38×/2 mM |
[27,28] |
| M-MSP |
ATTCGTTTCGTTTCGCGTTCGTTTC |
CGACTACAACTCCCGAACGATCCG |
60°C/34×/2 mM |
|
|
DAPK1
|
|
|
|
|
| U-MSP |
GGAGGATAGTTGGATTGAGTTAATGTT |
CAAATCCCTCCCAAACACCAA |
63°C/35×/1.5 mM |
[20] |
| M-MSP | GGATAGTCGGATCGAGTTAACGTC | CCCTCCCAAACGCCGA | 63°C/35×/1.5 mM | |
Abbreviations: M-MSP MSP for the methylated allele, U-MSP MSP for the unmethylated allele, Tm annealing temperature.