Skip to main content
. 2014 Feb 22;12:52. doi: 10.1186/1479-5876-12-52

Table 2.

Primer sequences and the reaction condition of DAPK1 and GAPDH Reverse transcription-polymerase chain reaction (RT-PCR)

Gene Forward primer (5’ to 3’) Reverse primer (5’ to 3’) Tm/cycles/MgCl 2 References
DAPK1
CAGTTTGCGGTTGTGAAGAA
CCTGCAACGAGTTCCAAGAT
53°C/35×/2 mM
[31]
GAPDH ACCACAGTCCATGCCATCACT TCCACCACCCTGTTGCTGTA 60°C/24×/2 mM [23]

Abbreviations: Tm annealing temperature.