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. Author manuscript; available in PMC: 2014 Mar 4.
Published in final edited form as: Prostate. 2013 Aug 6;73(15):1614–1622. doi: 10.1002/pros.22615

Fig. 3.

Fig. 3

RhoGDIα downregulates AR expression. A: Overexpression of RhoGDIα downregulates androgen receptor (AR) mRNA level in CWR22Rv1 cells in a dose-dependent manner. CWR22Rv1 cells were transfected with different amounts of RhoGDIα plasmids in FBS. Total RNAs were isolated for qRT-PCR analysis of AR mRNA expression. B,C: Overexpression of RhoGDIα inhibits AR protein expression in a dose-dependentmanner.CWR22Rv1 (B) or C4-2 (C) cells were transfected with different amounts of RhoGDIα in FBS condition. Total protein extracts were collected and subjected to Westernblot analysis. D: Overexpression of RhoGDIα inhibits AR promoter luciferase activity. CWR22Rv1 cells were co-transfected with AR promoter-driven luciferase reporter and RhoGDIα plasmids. Luciferase activity was measured.