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. 2014 Feb 19;12:14. doi: 10.1186/1741-7007-12-14

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Copyright © 2014 Ericson et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Localization of the seven small proteins essential in the procyclic life cycle stage. Anti-HA or anti-GFP antibodies were used to detect C-terminal HA- (Tb11.NT.28 and Tb11.NT.29) or GFP- (Tb10.NT.87, Tb10.NT.90, Tb10.NT.86, Tb3.NT.18, and Tb11.NT.108) tagged versions of the proteins by fluorescence microscopy. DNA was stained with Hoechst (blue) and DIC images are shown in the right panels. DIC, differential interference contrast.