Table 4.

Primer sets for identification and mutation of P48 gene in M. bovis strain Hubei-1

Primer	Sequence (5′–3′)	Aim
MB-F	GCTTCATGTGGTGATAAATACTTTA	Identify P48 gene
MB-R	CTATTTTTGTGTTTCTTTAGCCAAT
MB-a	CATGCCATGGGCGCTTCATGTGGTGATAAATACTTTA	Mutate point 192
MB-a’	TGAAATTTTATGAACTGCTTCCCAACCAGATTGATTG
MB-b	TCAATCAATCTGGTTGGGAAGCAGTTCATAAAATTTC	Mutate point 648
MB-b’	CAGAAACTGCTGGCCAAGGAATACCACCGAAGG
MB-c	CCTTCGGTGGTATTCCTTGGCCAGCAGTTTCTG	Mutate point 1275
MB-c’	CAGCAGGTTGGTCTGGCCATTTTGTACCTAAAGC
MB-d	CTTTAGGTACAAAATGGCCAGACCAACCTGCTGACC	Mutate point 1317
MB-d’	CTATTTTTGTGTTTCTTTAGCCAACCAGTTAATCATTTTC
MB-e’	GCTTCTGGGTGTTCTTTATTCCAGTCAATAATACCTTGG	Mutate point 699
MB-f	CATTCCAAGGTATTATTGACTGGAATAAAGAACACCC