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. 2014 Feb 18;45(1):20. doi: 10.1186/1297-9716-45-20

Figure 4.

Figure 4

Effect of mechanical damage of ALI cultures on BHV-1-GFP infection. BHV-1-GFP was applied to the apical surface of BAEC after the pseudostratified epithelium had been injured with a sterile needle. At 1 dpi, cells were stained for β-tubulin (A, C) to visualize cilia or (B) CD142 to detect basal cells (both in red). C) shows a vertical section of injured epithelium. Scale bars = 25 μm in (A), 100 μm in (B) and (C).