Fig. 6. Differential expression of Bcl-6 and Blimp-1 in DC subsets.
a) VENN diagrams of all transcription factors at least 2-fold differentially expressed by human intestinal SP vs DP DCs or by mouse SP vs DP DCs. Genes within the intersections showed conserved SP or DP-selective expression in mice and humans.
b) Microarray data for pdrm1 (Blimp1-encoding) and Bcl-6 genes in small intestinal SP versus DP DCs. Bar graphs show the mean and SEM of raw expression values.
c) BCL-6 staining on human intestinal DCs. hSP DC gates and data are shown in orange, hDP in blue, non-stained control in gray. Bar graph shows the MFI of BCL-6 staining in each subset (n=3, p<0.05 paired t test.
d) Expression of a YFP-Bcl-6 and a Prdm1-YFP reporter by gut associated mouse cDCs. Contour plots show gating strategies. Pre-gated on live, lineage negative (CD3, CD19, NK1.1), CD11chi, MHCIIhi cells. Histograms depict YFP-Bcl-6 expression in the indicated DC subsets (green: CD103−CD11b+, blue: mDP, orange: mSP) and a wildtype control (gray). Data are representative of two independent experiments (n=3).
e) MFIs for YFP-Bcl-6 and YFP-Prdm1 expressed in the indicated DC subsets in the mLNs and SI LP. Mean of three mice per group with SEM. *p=0.05, ***p<0.0009, ****p<0.0001; unpaired t-test.
f) For pLNs and spleen, histograms show expression of YFP-Bcl-6 and YFP-Prdm1 within CD8α+ cDCs (purple) and CD11b+ cDCs (light blue). Representative of 2 technical replicates, n=3