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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Nat Immunol. 2013 Dec 1;15(1):98–108. doi: 10.1038/ni.2768

Fig. 7. Bcl-6-deficient mice lack splenic CD8α+and intestinal mSP DCs.

Fig. 7

a) Plots show live-gated lineage-negative (CD3, CD19, NK1.1) mLN cells. Relative frequency of cDC (CD11c+, B220neg) versus pDC (CD11c+, B220pos) in wild type, Bcl-6 heterozygous, and Bcl-6-deficient mice. Representative of 4 biological replicates.

b) Plots show the relative abundance of CD11bsp, CD103+CD11b+ (mDP), and CD103+CD11b− (mSP) DC in mLNs, SI LP, and colon (gated on live, CD3, CD19, and NK1.1 negative, CD11c+, MHCIIhi cells). Representative of at least ten biological replicates.

c) Relative abundance of subsets defined by CD8α and CD11b in wild type versus Bcl-6 knockout mice in pLN and spleen. Gated on live, CD3, CD19, and NK1.1 negative, CD11c positive, MHC class II high cells. Representative of at least ten biological replicates.

d) Subset composition of cDC derived from wild type versus Bcl-6 knockout donor BM in mixed bone marrow chimeras. Left column plots show wild type donor derived DC, right column plots show Bcl-6 knockout donor-derived DC. Representative of three replicates (n=3).

e) Wild type and Bcl-6 knockout BM-derived cDC populations in pLN and spleen.

f) SI LP DC subset contribution of wild type and Bcl-6 knockout derived donor cells to mDP and mSP DC. Cells were gated on live, lineage negative (CD3, CD19, NK1.1), CD11c+, and MHC class II+. Bars show the mean contribution in percent to the defined subsets (mDP, mSP), wild type-derived cells in white, Bcl-6-derived cells in black. **p=0.002, unpaired t-test.