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. 1983 Jul;80(13):4149–4153. doi: 10.1073/pnas.80.13.4149

Monoclonal antibodies specific for Hantaan virus.

M C Franko, C J Gibbs Jr, P W Lee, D C Gajdusek
PMCID: PMC394218  PMID: 6408643

Abstract

Six hybridoma cell line producing monoclonal antibodies to Hantaan virus were established by fusion of NS-1 mouse myeloma cells with spleen cells of mice immunized with Hantaan virus strain 76-118. The specificity of these monoclonal antibodies was established by immunoblotting analysis and immunofluorescence. Five of the clones reacted with antigens on the cell surface and in the cytoplasm, and one clone reacted with a determinant expressed only in the cytoplasm of the infected cells. Two of the clones produced antibodies that reacted with a Mr 50,000 polypeptide in virus-infected cellular extracts and purified virus preparations. The monoclonal antibodies were used to examine the antigenic relationship among Hantaan virus strains and between Hantaan virus and Prospect Hill virus and the virus of nephropathia epidemica. Three antibodies were capable of distinguishing between the Lee strain and the 760-118 strain of Hantaan virus and three additional antibodies reacted with determinants shared by both virus strains. None of the six reacted with Prospect Hill virus or the virus of nephropathia epidemica.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Buchmeier M. J., Lewicki H. A., Tomori O., Oldstone M. B. Monoclonal antibodies to lymphocytic choriomeningitis and pichinde viruses: generation, characterization, and cross-reactivity with other arenaviruses. Virology. 1981 Aug;113(1):73–85. doi: 10.1016/0042-6822(81)90137-9. [DOI] [PubMed] [Google Scholar]
  2. Franko M. C., Koski C. L., Gibbs C. J., Jr, McFarlin D. E., Gajdusek D. C. Monoclonal antibody specific for myelin glycoprotein P0: derivation and characterization. Proc Natl Acad Sci U S A. 1982 Jun;79(11):3618–3622. doi: 10.1073/pnas.79.11.3618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. French G. R., Foulke R. S., Brand O. A., Eddy G. A., Lee H. W., Lee P. W. Korean hemorrhagic fever: propagation of the etiologic agent in a cell line of human origin. Science. 1981 Mar 6;211(4486):1046–1048. doi: 10.1126/science.6110243. [DOI] [PubMed] [Google Scholar]
  4. Kennett R. H., Denis K. A., Tung A. S., Klinman N. R. Hybrid plasmacytoma production: fusions with adult spleen cells, monoclonal spleen fragments, neonatal spleen cells and human spleen cells. Curr Top Microbiol Immunol. 1978;81:77–91. doi: 10.1007/978-3-642-67448-8_13. [DOI] [PubMed] [Google Scholar]
  5. Köhler G., Howe S. C., Milstein C. Fusion between immunoglobulin-secreting and nonsecreting myeloma cell lines. Eur J Immunol. 1976 Apr;6(4):292–295. doi: 10.1002/eji.1830060411. [DOI] [PubMed] [Google Scholar]
  6. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  7. Lee P. W., Amyx H. L., Gajdusek D. C., Yanagihara R. T., Goldgaber D., Gibbs C. J., Jr New hemorrhagic fever with renal syndrome-related virus in rodents in the United States. Lancet. 1982 Dec 18;2(8312):1405–1405. [PubMed] [Google Scholar]
  8. Lee P. W., Goldgaber D., Gibbs C. J., Jr, Gajdusek D. C., Yanagihara R. T., Svedmyr A., Hlaca D., Vesenjak-Hirjan J., Gligic A. Other serotypes of hemorrhagic fever with renal syndrome viruses in Europe. Lancet. 1982 Dec 18;2(8312):1405–1406. doi: 10.1016/s0140-6736(82)91309-5. [DOI] [PubMed] [Google Scholar]
  9. Lee P. W., Svedmyr A., Gajdusek D. C., Gibbs C. J., Jr, Nyström K. Antigenic difference between European and East Asian viruses causing haemorrhagic fever with renal syndrome. Lancet. 1981 Aug 1;2(8240):256–257. doi: 10.1016/s0140-6736(81)90505-5. [DOI] [PubMed] [Google Scholar]
  10. McCormick J. B., Sasso D. R., Palmer E. L., Kiley M. P. Morphological identification of the agent of Korean haemorrhagic fever (Hantaan virus)as a member of the Bunyaviridae. Lancet. 1982 Apr 3;1(8275):765–768. doi: 10.1016/s0140-6736(82)91812-8. [DOI] [PubMed] [Google Scholar]
  11. Norrby E., Chen S. N., Togashi T., Shesberadaran H., Johnson K. P. Five measles virus antigens demonstrated by use of mouse hybridoma antibodies in productively infected tissue culture cells. Arch Virol. 1982;71(1):1–11. doi: 10.1007/BF01315171. [DOI] [PubMed] [Google Scholar]
  12. Pruslin F. H., Scherer W. F. Intracellular localization of core and envelope proteins of Venezuelan encephalitis virus by immunofluorescence. Proc Soc Exp Biol Med. 1980 Sep;164(4):421–425. doi: 10.3181/00379727-164-40888. [DOI] [PubMed] [Google Scholar]
  13. Smith J. F., Pifat D. Y. Morphogenesis of sandfly viruses (Bunyaviridae family). Virology. 1982 Aug;121(1):61–81. doi: 10.1016/0042-6822(82)90118-0. [DOI] [PubMed] [Google Scholar]
  14. Svedmyr A., Lee H. W., Berglund A., Hoorn B., Nyström K., Gajdusek D. C. Epidemic nephropathy in Scandinavia is related to Korean haemorrhagic fever. Lancet. 1979 Jan;1(8107):100–100. doi: 10.1016/s0140-6736(79)90083-7. [DOI] [PubMed] [Google Scholar]
  15. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350–4354. doi: 10.1073/pnas.76.9.4350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. White J. D., Shirey F. G., French G. R., Huggins J. W., Brand O. M., Lee H. W. Hantaan virus, aetiological agent of Korean haemorrhagic fever, has Bunyaviridae-like morphology. Lancet. 1982 Apr 3;1(8275):768–771. doi: 10.1016/s0140-6736(82)91813-x. [DOI] [PubMed] [Google Scholar]

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