Figure 3.
Immunofluorescence multichromatic projections of PARKIN1, the nuclear factor Foxo3a, and the mitochondrial marker TOM20 in the ischaemic (IR) cardiac specimens after treatments with resveratrol or longevinex. Panels (a1) and (a2) are administration of vehicle only; panels (b1) and (b2) are administration of resveratrol; panels (c1) and (c2) are administration of longevinex, where: (1) are projections of PARKIN1 (green), Foxo3a (red), and nuclei (blue); nuclear localization of Foxo3a is indicated with white arrows in panels (b1) and (c1); (2) are projections of PARKIN1 (green), TOM20 (red), and nuclei (blue); and mitochondrial fission is indicated with white arrows in panels (b2) and (c2). The confocal images were taken with optical Z-step of 0.5 µm.