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. 2014 Feb 27;14:22. doi: 10.1186/1471-2415-14-22

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Copyright © 2014 Falk et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The CCR3 expression was measured using flow cytometry. The figure shows the gating strategy and quantification of CD9 positive granulocytes and their expression of CCR3. A, Granulocytes were identified based on CD45 expression and side scatter. B, the granulocytes were then gated based on the CD9 expression. C, the fraction of CCR3+ cells within the CD9+ granulocyte gate was calculated. Histogram showing the percentage of CD9 positive granulocytes positive for CCR3 (Light grey) and the negative isotype control set at 1% (dark grey).