Skip to main content
. 2004 Feb 26;23(8):1792–1803. doi: 10.1038/sj.emboj.7600138

Figure 4.

Figure 4

Effects of gcn5+ disruption on the genome-wide DSB formation and recombination. (A) Effects of gcn5Δ mutation upon meiotic DSBs in whole chromosomes. Genomic DNA was prepared from K341 (ade6-M26, rad50S, lanes WT) and TY24 (ade6-M26, rad50S, gcn5Δ, lanes gcn5Δ) cells cultured for the indicated times in sporulation medium, and was analyzed by pulsed-field gel electrophoresis. The electrophoresis image was obtained by an FM BIO II image analyzer (HITACHI) after Syber Green staining. The smeared DNA shows the broken DNA generated during meiosis. (B) Effects of the gcn5Δ mutation upon intergenic recombination. Standard genetic crosses between haploids containing appropriate markers were performed, and spores were plated on complete medium. Then the colonies arising from the spores were replicated onto appropriate test media to check the prototrophy and to measure the recombination frequencies. Each recombination frequency represents average of five independent crosses.