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. 2004 Mar 25;23(8):1729–1738. doi: 10.1038/sj.emboj.7600180

Figure 3.

Figure 3

Does EDC crosslink expressed and ‘native' myosin VI? (A) Purified myosin VI and myosin II HMM were treated with 5 mM EDC at RT and samples were taken at 30, 60 and 120 min and run on 3% acrylamide SDS-phosphate PAGE. Controls without EDC were left at RT for 120 min. Molecular weight markers (kDa) are indicated on the left of each gel. (B) NRK cells were grown to confluence and lysed. After centrifugation, the supernatant was incubated at RT with 50 mM EDC for 30 and 60 min or without EDC for 60 min. Samples were run on SDS- phosphate PAGE gels and blotted for myosin VI, myosin V and non-muscle myosin II. Molecular weight markers (kDa) are on the left.