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. 2014 Mar 4;9(3):e89894. doi: 10.1371/journal.pone.0089894

Figure 5. LLLT increased GAP-43 expression in a CCD model.

Figure 5

(A) The levels of GAP-43 mRNA expression were analyzed using the 2−ΔCT method and normalized to the Control group. (B) Fluorescence images of GAP-43 staining from the Control or CCD groups with or without LLLT were analyzed. Representative images from the Control (upper row), CCD (middle row) and CCD+LLLT (bottom row) groups are shown. GAP-43 signals are presented in the left column (green). Images of nuclear staining with DAPI are provided in the middle-left column (blue), and merged fluorescence images are shown in the middle-right column Magnified image of GAP-43 are provided in the right column (magnification 400×) and representative signals are indicated by arrowheads. Scale bars, 10 µm. (C) A graph displaying the quantification of GAP-43 fluorescence. The levels of statistical significance are as follows: *, p<0.05 and **, p<0.01 relative to the control group; #, p<0.05 and ##, p<0.01 relative to the CCD groups.