Table 2. Primer sequences used for Real-Time PCR.

Gene	Primer sequence
IL-1β	Forward: 5′-TCTCACAGCAGCATCTCGAC-3′
	Reverse: 5′-GGTCGTCATCATCCCACGAG-3′
TNF-α	Forward: 5′-GACCCCTTTATCGTCTACTCC-3′
	Reverse: 5′- GCAATCCAGGCCACTACTTC -3′
GAP43	Forward: 5′-GCCAAGGAGGAGCCTAAACA-3′
	Reverse: 5′-CTGCTTTCTGCAGTCTCCGT-3′
GAPDH	Forward: 5′-GAGAGAGGCCCTCAGTTGCCTGAGA-3′
	Reverse: 5′-GGCCCCTCCTGTTGTTATGGGG-3′

IL-1β: interleukin-1β; TNF-α: tumor necrosis factor alpha; GAP43: nerve growth-associated protein 43; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.