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. 2014 Mar 4;9(3):e89450. doi: 10.1371/journal.pone.0089450

Figure 3. The protective effect of GL on I/R-induced infarct volume and apoptosis injury is HMGB1 dependent.

Figure 3

A) Brain infarction induced by right MCA occlusion was evaluated by TTC staining from rats pre-treated with 4 mg/kg GL with or without 100 µg recombinant HMGB1, and the infarct volumes 48 h after reperfusion were quantified using NIH image software. B) Representative photomicrographs show TUNEL staining for apoptotic cells in rat brains at 48 h after reperfusion in the sham, NS, GL and GL+rHMGB1 groups. Effects on the severity of cerebral apoptosis are shown in an average quantitative analysis of the number of TUNEL-positive cells. C) Representative blots showing the effects of GL with or without rHMGB1 treatment on Cytochrome c translocation between the mitochondrial and cytosolic fractions. D) Effects of GL with or without rHMGB1 treatment on caspase-3 activity in areas at the risk zone of cerebral tissues at 48 h after reperfusion. Values are means±SEM, n = 8 for each group. *P<0.05, **P<0.01 (t test).