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. 2014 Mar 4;9(3):e89450. doi: 10.1371/journal.pone.0089450

Figure 5. The antioxidant effects of GL are HMGB1 dependent.

Figure 5

A) Fluorescent images after incubation with DHE showing superoxide levels in the I/R brain (red). Quantitative measurements of the fluorescence intensity indicated a significant change in the 4 mg/kg GL-treated group with or without rHMGB1, compared with the saline-treated I/R (NS) group. B) Representative photomicrographs showing 3-NT formation in the ischemic brain (green). Data from image analysis also indicated a significant change in the 4 mg/kg GL-treated group with or without rHMGB1, compared with the saline-treated I/R (NS) group. Bar = 50 µm. C) The effects of 4 mg/kg GL with or without rHMGB1 on MDA content in rat brains after I/R. Values are means±SEM, n = 8 for each group. *P<0.05, **P<0.01 (t test).