Skip to main content
. 2004 Apr 1;23(8):1782–1791. doi: 10.1038/sj.emboj.7600187

Figure 4.

Figure 4

X1 and X2 complexes can be separated in a glycerol gradient. A 150-μl crosslinking reaction performed in the presence of ATP and absence of U1-blocking DNA was separated in a 10–30% glycerol gradient. In all, 10 μl of each fraction was resolved in either a 4% native gel (A) or an 11% SDS gel (B). (C) Fractions 4 and 7 of the glycerol gradient were immunoprecipitated under native conditions using the indicated antibodies, and the bound material was resolved in an 11% SDS gel. The identity of the ∼70 kDa signal generated with the α-TLS antibody (lane 21) has not been determined. Positions of the 5′SS crosslinks are indicated.