Skip to main content
. 2014 Mar 4;9(3):e90220. doi: 10.1371/journal.pone.0090220

Figure 1. Downregulation of βig-h3 decreases adhesion, invasion and migration of human osteosarcoma cells in vitro.

Figure 1

(A) Western blot was performed to examine the βig-h3 protein levels in Saos-2 cells and MG63 cells which were transfected with control siRNA or βig-h3 siRNA. (B) Real Time PCR was performed to examine the βig-h3 mRNA levels in Saos-2 cells and MG63 cells which were transfected with control siRNA or βig-h3 siRNA. (C) The amounts of cell adhesion was tested in Saos-2 cells and MG63 cells which were transfected with control siRNA or βig-h3 siRNA. (D) The amounts of cell invasion was tested in Saos-2 cells and MG63 cells which were transfected with control siRNA or βig-h3 siRNA. (E) The amounts of cell migration was tested in Saos-2 cells and MG63 cells which were transfected with control siRNA or βig-h3 siRNA. Control siRNA were used as a negative control. Scale = 100 µm. The adhension assay, invasion assay, and migration assay were adopted as described in Materials and Methods. Values are the means±SE from six independent experiments. *P<0.05 by Student's t test.