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. 2014 Mar 4;9(3):e90220. doi: 10.1371/journal.pone.0090220

Figure 6. βig-h3 induces human osteosarcoma cells metastasis by activating PI3K signaling pathway.

Figure 6

(A) Expression levels of phosphorylated AKT (p-AKT) and AKT were analyzed in control siRNA or βig-h3 siRNA transfected Saos-2 cells. (B) Expression levels p-AKT and AKT were analyzed in control siRNA or βig-h3 siRNA transfected Saos-2 cells which were incubated with PI3K inhibitor LY294002. (C) Expression levels of p-AKT and AKT were analyzed in control siRNA or βig-h3 siRNA transfected Saos-2 cells which were incubated with P1E6 and 6S6, alone or combination. The amounts of cell adhesion (D), invasion (E) and migration (F) were tested in control siRNA or βig-h3 siRNA transfected Saos-2 cells which were incubated with LY294002. Scale = 100 µm. Bars represent the mean of triplicate samples; error bars represent standard deviation. Data are representative of three independent experiments. *P<0.05 by Student's t test.