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. 2014 Mar 4;9(3):e90250. doi: 10.1371/journal.pone.0090250

Figure 3. Ribbon-specific protein SV2B was downregulated in retina, but upregulated in IPL of rd1 mouse.

Figure 3

A) Representative blots of SV2B and β-tubulin in adult wild-type and rd1 mouse retinas. Each band is from a different animal. B) Ratio of SV2B to β-tubulin (mean ± SE) in adult rd1 mouse retina was significantly lower than in wild-type mouse (p = 0.01; n = 5). C) Relative mRNA levels (mean ± SE) of SV2B in adult rd1 mouse were approximately 40% lower than in wild-type, although the difference was not statistically significant (p = 0.062; n = 6). D, E) Representative images of retinal sections of adult wild-type (D) and rd1 (E) mouse retinas immunostained for SV2B. Scale bar: 50 µm. A) Intensity profile of SV2B through retinal depth of the images shown in D and E. Similar to synaptophysin (Figs. 2A, 2B, 2C), SV2B is nearly absent in OPL of rd1 mouse retina, whereas that in IPL is higher than in wild-type, particularly in the Off sublamina. B) Levels of SV2B (mean ± SE), measured with quantitative immunohistochemistry were more than twofold higher in IPL of adult rd1 mouse retina than in wild-type (p = 0.014; n = 5). The SV2B levels were higher in both On and Off sublaminas of IPL, although the increase was more pronounced in the Off sublamina (On: p = 0.031; Off: p = 0.005). *p<0.05; **p<0.01.