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. 2014 Mar 4;9(3):e90250. doi: 10.1371/journal.pone.0090250

Table 1. List of antibodies used.

Antibody Immunogen Manufacturer, Catalog no Species, type dilution
Synaptophysin A synaptosome preparation from rat retina Sigma-Aldrich (St. Louis, USA) cat. # S5768 Mouse monoclonal 1:5000 (Immunohistochemistry and Western blotting)
SV2B Synthetic peptide DDYRYRDNYEGYAPND (aa 2–17 in rat) coupled to key-hole limpet hemocyanin via an added N-terminal cysteine residue Synaptic systems (Goettingen, Germany) cat.#119111 Mouse Monoclonal 1:4000 (Immunohistochemistry); 1:12000 (Western blotting)
Syntaxin-I GST fusion protein corresponding to a cytoplasmic part of rat Syntaxin IA (residue 1–265) (Accession number P32851) Chemicon (Billerica, USA) cat. # AB5820-50UL Rabbit polyclonal 1:12000 (Western blotting)
Syntaxin Synaptic vesicle fractions that were immuo-precipitated from human brain homogenates using anti-human synaptophysin monoclonal antibodies Stressgen (Victoria, Canada) cat.# VAM-SV013 Mouse monoclonal 1:12000 (Western blotting)
Synapsin-I Synapsin I purified from bovine brain Sigma-Aldrich cat.# S193 Rabbit polyclonal 1:10000(Western blotting)
β-tubulin Tubulin from rat brain Sigma-Aldrich cat.# T4026 Mouse Monoclonal 1:12000 (Western blotting)