Figure 7. Egfl7 expression in mural cell deficient retinal vasculature.
A: Co-immunodetection of pericytes (NG2) and smooth muscle cells (SMA) in P9 retina from pups injected with vehicle or with the blocking antibody directed against the PDGFRβ (αPDGFRβ). B: Co-immunodetection at higher magnification of endothelial cells (isolectin B4), pericytes (NG2) and smooth muscle cells (SMA) in P9 retina from pups injected with vehicle or with the blocking antibody directed against the PDGFRβ (αPDGFRβ). C: Type IV collagen immunostaining showing the vasculature of P9 retina from pups injected with vehicle or with the blocking antibody directed against the PDGFRβ (αPDGFRβ). D: Combined collagen IV (Coll IV) immunostaining and egfl7 in situ hybridization in P9 retina from pups injected with vehicle or with the blocking antibody directed against the PDGFRβ (αPDGFRβ). a, artery; v, vein. Magnifications are indicated.