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Expression of early posterior markers is unaffected in Tcf4−/−/Tcf1−/− embryos. Whole-mount in situ hybridizations of E8.5–9.5 littermates using various posterior markers (see above). (A, C, E, G, I) Tcf4+/−/Tcf1−/− embryos and (B, D, F, H, J) Tcf4−/−/Tcf1−/− embryos. Panels (A, B) and (C, D) represent E8.5 and E9.5 embryos, respectively, and show that initiation and maintenance of T expression is unchanged in Tcf4−/−/Tcf1−/− embryos.
