Table 1. Upregulation of genes sensitive to Pitx2 dosage in the piglet DHF model.

Gene coding for	Gene symbol	Fold change		PITX2 binding to the promoter	Reference
		microarray	qRT-PCR
Paired-like homeodomain 2	Pitx2	2.14	6.60±1.6*
Natriuretic peptide precursor A	Nppa	4.42	HV	+	[73]
Cyclin D2	Ccnd2	2.38	HV	+	[51]
Forkhead box protein J1	Foxj1	2.66	3.20±0.4	+	[74]
Myogenic factor 5	Myf5	5.15	4.80±0.5	+	[57]
Paired box 3	Pax3	3.39	1.80±0.3	+	[45]

Three pairs of failing-non failing samples were assayed by both microarray and qRT-PCR. *Quantitative RT-PCR analysis with the use of the primer pair (350–353, see Table S3) annealing to the sequence common to the different Pitx2 variants. HV – highly variable expression within each sample sets.