Table 1. Upregulation of genes sensitive to Pitx2 dosage in the piglet DHF model.
Gene coding for | Gene symbol | Fold change | PITX2 binding to the promoter | Reference | |
microarray | qRT-PCR | ||||
Paired-like homeodomain 2 | Pitx2 | 2.14 | 6.60±1.6* | ||
Natriuretic peptide precursor A | Nppa | 4.42 | HV | + | [73] |
Cyclin D2 | Ccnd2 | 2.38 | HV | + | [51] |
Forkhead box protein J1 | Foxj1 | 2.66 | 3.20±0.4 | + | [74] |
Myogenic factor 5 | Myf5 | 5.15 | 4.80±0.5 | + | [57] |
Paired box 3 | Pax3 | 3.39 | 1.80±0.3 | + | [45] |
Three pairs of failing-non failing samples were assayed by both microarray and qRT-PCR. *Quantitative RT-PCR analysis with the use of the primer pair (350–353, see Table S3) annealing to the sequence common to the different Pitx2 variants. HV – highly variable expression within each sample sets.