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. 2004 Apr 8;23(8):1761–1769. doi: 10.1038/sj.emboj.7600193

Figure 2.

Figure 2

S6K1 phosphorylates eIF4B in vitro on a phosphopeptide comigrating with the endogenous serum-stimulated phosphopeptide 2. (A) Recombinant eIF4B was labeled in vitro with S6K1; or (B) immunoprecipitated from 32P-labeled 293 cells, and subjected to phosphopeptide mapping. (C) The in vitro- and in vivo-labeled samples were then mixed, and subjected to phosphopeptide mapping.